The Basis of Sanger DNA Sequencing Is to

question 23

Multiple Choice

The basis of Sanger DNA sequencing is to

• A) identify ESTs and place them in order based on their function to derive the overall sequence.
• B) collect all of the mRNAs of a given cell type, make cDNAs from them, align the cDNAs, and derive the overall sequence.
• C) break several copies of a genome into 3-base codons, and align them to derive the overall sequence.
• D) generate a series of fragments from an overall DNA sequence that differ by the end base of each. Overlap the pieces and read off the overhangs to derive the sequence.
• E) tag each of the four DNA bases with a fluorescent marker molecule and align them by the intensity of the fluorescence to derive the overall sequence.

Identify how to adjust and apply overhead based on actual and estimated figures.

Comprehend the procedure for recording the use of raw materials in production.

Understand how to calculate the total manufacturing cost added to work in process during the year.

Understand the concept of manufacturing overhead and its application based on different bases like direct labor-hours and machine-hours.

---

Definitions:

Club Fungi

A group of fungi characterized by the production of spores on club-shaped structures called basidia.

Ascomycota

Ascomycota, also known as sac fungi, is a division of fungi that store their spores in a sac-like structure called an ascus.

Basidiomycota

A phylum of fungi characterized by producing spores on basidia, often found as mushrooms, toadstools, smuts, rusts, and puffballs.

Black Bread Mold

A common type of fungus that grows on bread and other food items, causing spoilage.