Which of the Following Sequences of Events in the Production

question 35

Multiple Choice

Which of the following sequences of events in the production of recombinant DNA are in the correct order?

• A) mix DNA with plasmid DNA, cut with restriction enzymes, add DNA ligase
• B) add DNA ligase, cut DNA with restriction enzymes, mix DNA with plasmid DNA
• C) cut DNA with restriction enzymes, mix DNA with plasmid DNA, add DNA ligase
• D) mix DNA with plasmid DNA, add DNA ligase, cut with restriction enzymes
• E) cut with restriction enzymes, add DNA ligase, mix DNA with plasmid DNA

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Definitions:

Dependent Variable

The variable in an experiment or study that is expected to change in response to changes in another variable known as the independent variable.

Independent Variable

A variable in an experiment that is manipulated or changed by the researcher to observe its effects on the dependent variable.

Least Squares Estimate

A statistical method used to minimize the sum of square differences between observed and estimated data points.

Dependent Variable

A variable in an experiment or model that is expected to change in response to changes in other variables.