The Steps Involved in a Single PCR Cycle, in the Correct

question 15

Multiple Choice

The steps involved in a single PCR cycle, in the correct order, are

• A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
• B) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
• C) heat to denature DNA strands, cool to allow primer to anneal and to allow DNA polymerase to copy DNA.
• D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
• E) cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.

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Definitions:

Confounding

In research and statistics, describes a situation where the effect of the independent variable on the dependent variable is mixed with the effect of another variable, complicating the analysis and interpretation of results.

Experimental Effects

The changes or outcomes observed in an experimental setting, attributable to the manipulation of independent variables.

Autonomy

The capacity to make independent choices and to have self-governance, often discussed in the context of moral, ethical, or political theory.

Differences Tradition

is a concept within cultural studies that emphasizes understanding and appreciating the diverse ways people differ from one another, including cultural, gender, racial, and ethnic differences.