In a Polymerase Chain Reaction,the Threshold Cycle Represents

question 3

Multiple Choice

In a polymerase chain reaction,the threshold cycle represents:

• A) the minimum amount of target that can be amplified.
• B) an indication of the original amount of target in the sample.
• C) the maximum number of cycles that can be carried out before the amplicons saturate the solution.
• D) the degree of complementarity between the primers and the target.