When Selecting a Photometric Technique That Is More Sensitive to a Low

question 13

Multiple Choice

When selecting a photometric technique that is more sensitive to a low concentration of analyte,a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity.Which technique is considered more sensitive in its measurements and why?

• A) Absorbance measurements are more sensitive because of the ability of the monochromator to isolate very specific parts of the spectrum.
• B) Fluorescence intensity measurements are more sensitive because the fluorophores used are very specific to the analytes they bind to and will bind to small analytes easily.
• C) Fluorescence intensity measurements are more sensitive because of the use of intense light sources,signal filtering,and sensitive light emission photometers.
• D) Absorbance measurements are more sensitive because the flame can break down complex compounds into elements that are specifically measured.

---

Definitions:

Sodium Sulfate

An inorganic salt used in detergents, paper manufacturing, and glass production, known chemically as Na2SO4.

Atoms

Atoms are the basic units of matter and the defining structure of elements, consisting of a nucleus surrounded by electrons, and classified by the number of protons they contain.

Washing Detergent

A chemical substance used to break up and remove dirt and grease from fabrics, commonly in the form of powder or liquid.

Calcium Nitrate

An inorganic compound used as a nitrogen fertilizer in agriculture and also in waste water pre-conditioning for odor emission prevention.