There Are Several Interferences That Can Affect or Limit Fluorescence

question 9

Multiple Choice

There are several interferences that can affect or limit fluorescence measurements.For example,the inner filter effect:

• A) occurs when the solution absorbs greater than 2% of the exciting light,producing a nonlinear response between concentration and fluorescence emission.
• B) involves fluorophores that have overlapping excitation and emission spectra and that are susceptible to loss of detection because of background light scatter.
• C) involves solvents such as ethanol that cause appreciable fluorescence due to the interaction of the fluorophore with the solvent.
• D) is caused by light scattering of proteins and other macromolecules in the sample matrix,which might cause unwanted background fluorescence.

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