When Selecting a Photometric Technique That Is More Sensitive to a Low

question 13

Multiple Choice

When selecting a photometric technique that is more sensitive to a low concentration of analyte,a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity.Which technique is considered more sensitive in its measurements and why?

• A) Absorbance measurements are more sensitive because of the ability of the monochromator to isolate very specific parts of the spectrum.
• B) Fluorescence intensity measurements are more sensitive because the fluorophores used are very specific to the analytes they bind to and will bind to small analytes easily.
• C) Fluorescence intensity measurements are more sensitive because of the use of intense light sources,signal filtering,and sensitive light emission photometers.
• D) Absorbance measurements are more sensitive because the flame can break down complex compounds into elements that are specifically measured.

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