Why Do Values Obtained with the Jaffe Reactions for Creatinine

question 2

Multiple Choice

Why do values obtained with the Jaffe reactions for creatinine measurement overestimate creatinine concentration by ~20%?

• A) The components of the reaction mixture often continue to react at endpoint and synthesize excess creatinine.
• B) The rate of Jaffe complex formation is temperature dependent,and excess heat formed by the reaction leads to increased absorptivity and falsely increased creatinine value.
• C) Noncreatinine chromogens in the reaction that react with the picrate in the end-point Jaffe methods overestimate true plasma creatinine concentration.
• D) Hydroxide concentrations above 0.5 mmol/L produce a decreased degradation of the Jaffe complex.

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Definitions:

Alpha

Often referred to as the level of significance in hypothesis testing, representing the threshold for rejecting the null hypothesis.

Non-directional

A type of hypothesis that does not specify the direction of the expected difference or relationship.

Alternative Hypothesis

A hypothesis that contradicts the null hypothesis, suggesting that there is a significant effect or difference.

Inferential Statistics

Statistical procedures used to test hypotheses and draw conclusions from data collected during research studies.