You Have Prepared a Restriction Enzyme Digest of DNA from an Individual

question 24

Multiple Choice

You have prepared a restriction enzyme digest of DNA from an individual who might have a genetic disorder.On the gel,you note that the digest from this individual produces one fragment,whereas the digest from a normal healthy individual produces two fragments.All controls worked correctly and you used the same reagents for all testing.What is your interpretation?

• A) The enzymes were not working.
• B) The diseased individual's DNA is missing a restriction site.
• C) You must have skipped the amplification step.
• D) The normal individual's DNA is missing a restriction site.

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