If RNA Is to Be Used in a PCR Amplification

question 11

Multiple Choice

If RNA is to be used in a PCR amplification procedure,what is the initial step that must be performed?

• A) The RNA must be denatured to form single strands for annealing to primers.
• B) RNA should never be used in a PCR reaction because thermostable enzymes cannot synthesize strands to anneal to it.
• C) A reverse transcription procedure must be performed to form cDNA.
• D) RNA must first be treated with RNases to remove interfering substances from the target.

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