Which PCR Step Causes the Denaturation of Double-Stranded DNA

question 5

Multiple Choice

Which PCR step causes the denaturation of double-stranded DNA?

• A)Add DNA polymerase and nucleotides at 72°C.
• B)Cool DNA to between 50°C and 65°C.
• C)Add primers.
• D)Heat target DNA to 94°C.
• E)Repeat the cycle of heating and cooling.

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Definitions:

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Nonadherence

The act of not following prescribed guidelines, advice, or directives, commonly used in reference to medical treatment plans or dietary recommendations.

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The action of consuming the first meal of the day, traditionally eaten in the morning, considered significant for energy and nutrient intake.

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A condition characterized by an abnormally low level of glucose (sugar) in the blood, often leading to symptoms such as shakiness, sweating, confusion, and in severe cases, unconsciousness.