When Determining the Sequence of Nucleotides in an Unknown Sample

question 20

Multiple Choice

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

• A)PCR
• B)Cloning
• C)The Sanger Method
• D)Southern blot hybridization
• E)Microarray analysis

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Definitions:

Spin-Spin Splitting

A phenomenon in NMR spectroscopy where a nucleus's magnetic field is split by the magnetic fields of neighboring nuclei.

Chemical Shift

A term used in nuclear magnetic resonance (NMR) spectroscopy to describe the change in magnetic resonance frequency of a nucleus due to its chemical environment.

Sample Proportions

The fraction or percentage of the sample that represents a particular trait or characteristic of interest.

Control Chart

A graphical tool for monitoring process stability and variability by plotting measured values against predetermined control limits.