Which PCR Step Causes the Denaturation of Double-Stranded DNA

question 62

Multiple Choice

Which PCR step causes the denaturation of double-stranded DNA?

• A)add DNA polymerase and nucleotides at 72°C
• B)cool DNA to between 50°C and 65°C
• C)add primers
• D)heat target DNA to 94°C
• E)repeat the cycle of heating and cooling

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