A Protein with Five Disulfide Bonds Was Treated With $\beta$ -Mercaptoethanol and Urea

question 11

Multiple Choice

A protein with five disulfide bonds was treated with $\beta$ -mercaptoethanol and urea. Once the protein was denatured, the $\beta$ -mercaptoethanol and urea were removed by dialysis. What is the likelihood that all five disulfide bonds will reform correctly?

• A)1/5 or 20%
• B)1/10 or 10%
• C)1/10² or 4%
• D)1/5! = 1/120 or 0.83%
• E)1/945 or 0.11%

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