A Protein with Five Disulfide Bonds Was Treated With $\beta$ -Mercaptoethanol and Urea

question 11

Multiple Choice

A protein with five disulfide bonds was treated with $\beta$ -mercaptoethanol and urea. Once the protein was denatured, the $\beta$ -mercaptoethanol and urea were removed by dialysis. What is the likelihood that all five disulfide bonds will reform correctly?

• A)1/5 or 20%
• B)1/10 or 10%
• C)1/10² or 4%
• D)1/5! = 1/120 or 0.83%
• E)1/945 or 0.11%

---

Definitions:

Granulated Tissue

New connective tissue and microscopic blood vessels that form on the surfaces of a wound during the healing process.

Minimal Scarring

The least possible formation of fibrous tissue (scar) at the site of an injury or incision, often a goal in surgical healing.

Braden Scale

A tool used to predict the risk of developing pressure ulcers by evaluating factors such as mobility, moisture, activity, nutrition, friction, and shear.

Pressure Ulcer Risk

Refers to the likelihood of developing bed sores due to prolonged pressure on the skin, commonly assessed in immobilized patients.