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Many Enzyme-Catalyzed Reactions That Transfer a Phosphate from One Molecule

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Essay

Many enzyme-catalyzed reactions that transfer a phosphate from one molecule to another utilize a histidine residue in the active site of the enzyme. The histidine participates in covalent catalysis by performing a nucleophilic attack on a phosphate, forming an intermediate phosphohistidine (the phosphate covalently attached to the histidine residue). Using ATP as the phosphate donor, show the reaction to form the phosphohistidine intermediate-you do not have to show arrow pushing.


Definitions:

Independent Variable

In an experimental setup, the variable that is manipulated by the researcher to examine its effect on the dependent variable.

Confounded Variable

A factor other than the independent variable that might cause or influence the outcome of an experiment, potentially biasing the results.

Operational Definition

A description of a concept or variable in terms of the specific process or set of validation tests used to determine its presence and quantity.

Hypothesis

A proposed explanation for a phenomenon, intended to be tested through research.

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