Why Must an Engineered DNA Polymerase Be Used in PCR

question 31

Multiple Choice

Why must an engineered DNA polymerase be used in PCR and not the one that occurs naturally?

• A) It needs to work at very cold temperatures.
• B) It needs to attach to DNA without a primer.
• C) It must work in the 3' to 5' direction adding nucleotides to the 5' end.
• D) It must work on single stranded DNA at high temperatures and attach to an oligonucleotide primer.
• E) It must attach without a primer, work in the 3' to 5' direction and function at very cold temperatures.

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Definitions:

Cell Division

The process by which a parent cell divides into two or more daughter cells, allowing for growth and the replacement of worn-out cells.

Dermal Tissue System

A complex of tissues in plants that forms the outer protective covering, performing functions such as protection, water regulation, and gas exchange.

Epidermis

The outermost layer of cells that covers the body of an organism, providing protection against the environment.

Periderm

A protective tissue in plants that replaces the epidermis in parts that undergo secondary growth, consisting of the cork, cork cambium, and phelloderm.