Ion Exchange Chromatography Is a Commonly Used Method for Separation

question 68

Essay

Ion exchange chromatography is a commonly used method for separation of biomolecules.
a.What are the two types of ion exchange chromatography?
b.In what order would Arg,Val,and Glu elute from a carboxymethyl column at pH 6.0.Carboxymethyl is negatively charged at pH 6.0.
c.You are trying to purify a protein using ion exchange chromatography.Unfortunately,your protein remains bound to the ion exchange column or it is eluting very slowly.What are the two changes you can make,to try to elute your protein more quickly from this column?

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