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You have purified the receptor for a hormone by affinity chromatography.During gel filtration chromatography under native conditions the receptor elutes between pyruvate decarboxylase (250 kDa)and glutamine synthetase (620 kDa).During SDS-PAGE,in the absence of reducing agents,the receptor migrates as a single band of approximately 230 kDa.When SDS-PAGE is carried out in the presence of 2-mercaptoethanol the receptor migrates as two bands of approximately 95 and 135 kDa.Explain this result.
Stage 1 Sleep
The initial stage of the sleep cycle, characterized by light sleep, easy awakenings, and a reduction in bodily movement and heart rate.
Brain Wave Activity
The patterns of electrical signals from the brain, commonly measured by EEG, indicating different states of arousal and consciousness.
Higher Amplitude
Refers to waves with greater energy and intensity, typically seen in contexts like sound, light, or brain activity measurements.
Slower Frequency
A rate of occurrence that is reduced or less frequent, typically used in the context of waveforms or signals.
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