DNA Sequencing by the Chain-Termination Method Utilizes DNA Polymerase I

question 1

Multiple Choice

DNA sequencing by the chain-termination method utilizes DNA polymerase I to make a complementary copy of the target or template DNA molecule.A reaction with a 20 bp template and dideoxyadenosine nucleotides as terminators results in the production of a 5 bp fragment.Based on this result,we can conclude that the template contains

• A)a cytosine at position 5.
• B)a thymine at position 5.
• C)a cytosine at position 16.
• D)a thymine at position 16.
• E)a uracil at position 5.

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