In Affinity Labeling,a Technique Used to Study Enzyme Mechanisms

question 53

Multiple Choice

In affinity labeling,a technique used to study enzyme mechanisms,

• A)A compound designed to bind at the active site reacts with and therefore permits the identification of a nearby group.
• B)A fluorescent group is attached to the substrate to identify the position of the active site.
• C)Highly purified enzyme is obtained by affinity chromatography for mechanistic studies.
• D)A radioactive isotope is incorporated into the substrate to identify the position of the active site.
• E)Genetic modification of amino acid residues is used to alter the binding affinity of the active site.

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