An Extremely Efficient Enzyme Called "Efficase" Catalyzes the Conversion of "A

question 47

Multiple Choice

An extremely efficient enzyme called "efficase" catalyzes the conversion of "A" to "B." A researcher decides to mutate the enzyme in order to try to improve its performance.Following active site mutations,a significant reduction in the value of K_M and V_max was observed.Which of the following may have occurred?

• A)The affinity of the enzyme for the substrate was increased to a point which did not favor propagation (continuation)of the reaction.
• B)The decrease in V_max was not related to the decrease in K_M.
• C)If the reaction was first-order,the change in K_M cannot have affected V_max.
• D)The stability of E+S (E+A as written above)was increased,thereby increasing the K_M.
• E)The reverse reaction (breakdown of EA to E+A)was favored,slowing the V_max.

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