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Which of the following best explains why an enzyme hasn't evolved to catalyze the following reaction as the first step of glycolysis?
glucose + Pi -> glucose-6-phosphate
I.The use of ATP is required for this step to serve as a regulatory control point.
II.The use of ATP is important because it helps keep its concentration low.Otherwise the high [ATP] would slow glycolysis too much through feedback inhibition.
III.The large positive G˚' of this reaction would need to be overcome by an exceedingly high [Pi].
IV.Inorganic phosphate cannot be specifically placed on the carbon in the 6th position.
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