In a Transformation Experiment,DNA of a P⁺q⁺ Strain Was Used

question 10

Essay

In a transformation experiment,DNA of a p⁺q⁺ strain was used to transform a recipient strain that is auxotrophic for these markers.The number of each class of transformants is shown below:
$$\begin{array} { | c | c | c | } \hline \text { Class } & \text { Genotype } & \text { Number } \\\hline 1 & \mathrm { p } ^ { + } \mathrm { q } ^ { + } & 405 \\\hline 2 & \mathrm { p } ^ { + } \mathrm { q } ^ { - } & 300 \\\hline 3 & \mathrm { p } ^ { - } \mathrm { q } ^ { + } & 195 \\\hline\end{array}$$
a)If the total number of transformants isolated was 900,what is the co-transformation frequency for the p and q loci?
b)Use diagrams to show how each class of transformants were obtained starting with the recipient auxotroph.

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