You Found a Mouse Gene Sequence Containing an Open Reading

question 15

Essay

You found a mouse gene sequence containing an open reading frame that should produce a 43 kDa protein.You express this protein in cultured mouse cells by transforming the cells with a plasmid bearing your gene of interest.When you isolate proteins from these cells and probe for your protein of interest,you find that the detected protein is 49 kDa.Why might your protein be larger than expected when analyzed by immunoblot?

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Definitions:

Physical Agents

Natural or manufactured sources of energy such as heat, light, sound, and radiation, used therapeutically to treat various medical conditions.

Physical Medicine

A branch of medicine focused on the diagnosis, treatment, and prevention of disease and disabilities using physical methods such as exercises, massage, and other therapies.

Diseases

Medical conditions characterized by specific signs and symptoms that affect the body's structure or function.

Nervous System

The complex network of nerves and cells that transmits signals between different parts of the body, coordinating its actions and sensory information.