You Found a Mouse Gene Sequence Containing an Open Reading

question 15

Essay

You found a mouse gene sequence containing an open reading frame that should produce a 43 kDa protein.You express this protein in cultured mouse cells by transforming the cells with a plasmid bearing your gene of interest.When you isolate proteins from these cells and probe for your protein of interest,you find that the detected protein is 49 kDa.Why might your protein be larger than expected when analyzed by immunoblot?

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Definitions:

Logistics Customer Service

Part of the supply chain management that plans, implements, and controls the efficient, effective forward and reverse flow and storage of goods, services, and related information between the point of origin and the point of consumption in order to meet customer's requirements.

Marketing Perspective

An approach to business focusing on understanding and satisfying the needs and wants of customers through market research and strategic promotion.

Customer Relationship Management

A strategy for managing an organization's interactions with current and future customers, using data analysis about customers' history with a company to improve business relationships.

Stockout

A situation where inventory is exhausted and items are unavailable for sale or use, often resulting in lost sales or delays.