Which of the Following Statements About PCR Is/are FALSE

question 3

Multiple Choice

Which of the following statements about PCR is/are FALSE?

• A)PCR reaction contains DNA template,primers,deoxyribonucleotide triphosphates,and heat-resistant DNA polymerase.
• B)Each PCR cycle includes three steps: denaturation,annealing,and extension.
• C)After each PCR cycle,amount of DNA increases in a linear manner.
• D)One limitation in PCR is that it requires prior knowledge of the sequence or at least part of the sequence to be amplified.
• E)PCR is a very sensitive technique and can amplify target sequences present in extremely low copy numbers.

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