The Restriction Enzyme ClaI Recognizes the Sequence AT $\times$ CGATIt Cleaves the Phosphodiester Backbone Between the T and the Cleaves

question 9

Essay

The restriction enzyme ClaI recognizes the sequence AT $\times$ CGAT.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.The restriction enzyme TaqI recognizes the sequence T ↓ CGA.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.
a)Indicate the ends of the double-stranded DNA molecule left after cleavage by ClaI.Show the sequence,polarity,and overhang,if any.
b)On average,how many sites would TaqI recognize in a random sequence of 1200 base pairs?
c)If a TaqI-digested DNA end is ligated to a ClaI-digested DNA end,will ClaI cleave the newly ligated DNA? Will TaqI cleave the newly ligated DNA?

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Premium

The amount paid for an insurance policy or an amount paid above the nominal value for a security; also, the fees paid for an option contract.

Protective Put

Purchase of an asset combined with a put option on that asset to guarantee proceeds at least equal to the put’s exercise price.

Underlying Asset

The financial asset upon which a derivative instrument, such as a futures or options contract, is based.

Convertible Bond

A bond with an option allowing the bondholder to exchange the bond for a specified number of shares of common stock in the firm. The conversion ratio specifies the number of shares. The conversion price is the current value of the shares for which the bond may be exchanged. The conversion premium is the excess of the bond’s value over the conversion value.