You Constructed a CDNA Library in a Lambda Vector Using

question 42

Essay

You constructed a cDNA library in a lambda vector using mRNAs from a pea plant.You want to identify a rubisco (a protein involved in photosynthesis)clone among the 50,000 clones in the library.You have a rubisco cDNA from tobacco to use as a probe.When hybridizing the probe to the plaques in the library,would you use higher,lower,or the same temperature as for hybridizing the tobacco DNA to a tobacco rubisco clone? Why?

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Definitions:

Infant

A very young child or baby, typically under one year of age, at the early stages of development.

New York Longitudinal Study

The New York Longitudinal Study is a research project that tracked the developmental characteristics of children from infancy into adulthood to understand personality development.

Temperaments

The combination of mental, physical, and emotional traits of a person; often considered innate or inherent to one's character.

Infants

refer to young children in the earliest stage of their lives, typically considered to be from birth to one year old.