The Intermediate Temperature Cycle (72°C)in the Polymerase Chain Reaction Enables

question 31

Multiple Choice

The intermediate temperature cycle (72°C) in the polymerase chain reaction enables:

• A)hybridization between the template DNA and the PCR primers.
• B)denaturation of the template DNA to enable primer access to matching sequence.
• C)activation of the primers to being the amplification procedure.
• D)activity of the thermostable DNA polymerase enzyme.
• E)a reduction of contamination in the amplification reaction.

---

Definitions:

Expected Time

The estimated time assumed for the completion of a task or project, often used in planning and scheduling.

Optimistic

A mindset or attitude that tends to expect the most favorable outcomes of situations or believes that good ultimately predominates over evil in the world.

Pessimistic

A tendency to anticipate unfavorable outcomes or emphasize the negative aspects of a situation.

Expected Time

An estimation of the duration it would likely take to complete a task, often used in project management for scheduling.