Restriction Endonucleases BamH1,NheI,XbaI,and EcoRI Make Sequence-Specific Cuts in DNA at the Following

question 26

Essay

Restriction endonucleases BamH1,NheI,XbaI,and EcoRI make sequence-specific cuts in DNA at the following sequences:
BamHI: $\quad$ 5'-GGATCC-3'
NheI: $\quad$ $\quad$ 5'-GCTAGC-3'
XbaI: $\quad$ $\quad$ 5'-TCTAGA-3'
EcoRI: $\quad$ $\quad$ 5'-GAATTC-3'
All these enzymes break a phosphodiester bond between the first and second nucleotide (counting from the 5' end),leaving a 5' P and a 3' OH.
Could ligase covalently join two ends produced by the following enzymes? If so,would ligation regenerate one of the above restriction sites?
(1)two BamH1 ends?
(2)two XbaI ends?
(3)a BamH1 and an EcoRI end?
(4)an XbaI and an NheI end?
(5)a BamH1 and an NheI end?

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Definitions:

Neocortical Processing

Relates to the functions of the neocortex, a part of the brain involved in higher-order brain functions such as sensory perception, cognition, and motor control.

Temporal Processing

The brain's ability to perceive and organize stimuli or events in time, including the capacity to sequence, compare, and predict temporal relationships.

Cranial Nerves

Twelve pairs of nerves that emerge directly from the brain, not the spinal cord, and supply the head and neck with motor and sensory innervation.

Neocortex

The most recently evolved, outer layer of the cerebral cortex that plays a key role in higher brain functions such as sensory perception, cognition, and motor control.