Examlex
The restriction enzyme ClaI recognizes the sequence AT CGAT.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.The restriction enzyme TaqI recognizes the sequence T ↓ CGA.It cleaves the phosphodiester backbone between the T and the C bases at the arrow.
a)Indicate the ends of the double-stranded DNA molecule left after cleavage by ClaI.Show the sequence,polarity,and overhang,if any.
b)On average,how many sites would TaqI recognize in a random sequence of 1200 base pairs?
c)If a TaqI-digested DNA end is ligated to a ClaI-digested DNA end,will ClaI cleave the newly ligated DNA? Will TaqI cleave the newly ligated DNA?
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