Provide the sequence of reagents that are needed to accomplish the multistep synthesis below. 
Definitions:
Degrees Of Freedom
Represents the count of values in a calculation that are free to vary without infringing on preset constraints.
Test Statistic
A value calculated from sample data that is used in a hypothesis test to decide whether to reject the null hypothesis.
T Test
A statistical hypothesis test used to compare the means of two groups and determine if they come from the same population by analyzing the difference.
T Value
A value calculated in a t-test that assesses the difference between two means relative to the variation in the data.
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