You Are Given a Mixture That Contains Glutamic Acid (PI

question 94

Essay

You are given a mixture that contains glutamic acid (pI = 3.2),arginine (pI = 10.8),and valine (pI = 6.0),and you subject the mixture to electrophoresis.
a)Which amino acids migrate toward the cathode when the electrophoresis is carried out at a pH of 7.1?
b)Which amino acids migrate toward the anode when the electrophoresis is carried out at a pH of 7.1?
c)Which amino acid migrates farthest toward the anode at a pH of 7.1?
d)Since amino acids themselves are colorless,how is the separation of amino acids after electrophoresis detected?

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