You Have Purified the Receptor for a Hormone by Affinity

question 38

Essay

You have purified the receptor for a hormone by affinity chromatography.During gel filtration chromatography under native conditions the receptor elutes between pyruvate decarboxylase (250 kDa)and glutamine synthetase (620 kDa).During SDS-PAGE,in the absence of reducing agents,the receptor migrates as a single band of approximately 230 kDa.When SDS-PAGE is carried out in the presence of 2-mercaptoethanol the receptor migrates as two bands of approximately 95 and 135 kDa.Explain this result.

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Definitions:

Cash Basis

An accounting method where revenues and expenses are recorded when cash is received or paid, not when the obligation is incurred.

Comparability

An accounting principle that ensures financial statements can be compared between periods and across companies.

Financial Statements

Consolidated reports that summarize the financial performance, position, and cash flows of a business over a specified period.

Adjusting Entries

Journal entries made in accounting to adjust income and expense accounts so they reflect the true financial position of a business.