DNA Fragments Are Usually Inserted into a Plasmid Vector and Cloned

question 28

Multiple Choice

DNA fragments are usually inserted into a plasmid vector and cloned before they are analyzed by chain termination sequencing because

• A)DNA fragments need to be pure in order to obtain an unambiguous sequence.
• B)a large number of identical fragments is needed in a sequencing reaction in order to generate detectable amounts of products.
• C)plasmid vectors have known sequences that can be used for design and annealing of the primer oligonucleotides,which are used to initiate the sequencing reaction.
• D)all of the above
• E)none of the above

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Definitions:

Sedimentary Environments

Various geographical settings where sediment accumulation occurs, significantly influencing the formation and type of sedimentary rocks.

Vancouver

A coastal seaport city in western Canada, known for its vibrant culture and natural beauty.

Landslides

Landslides refer to the movement of soil, rock, and debris down a slope due to gravity, often triggered by rain, earthquakes, or human activity.

Lithifies

The process by which sediments are compacted and cemented into solid rock, typically over geological time scales.