Native Protein Purifications Often Require Multiple Reaction Steps in Order

question 37

Multiple Choice

Native protein purifications often require multiple reaction steps in order to purify the protein of interest from other proteins.One method used for protein separation in purification procedures is a change from water to an organic solvent.Which of the following would be accomplished by this solvent change?

• A)Proteins with hydrophobic groups on the interior would maintain their native state.
• B)Proteins with hydrophilic groups on the exterior would denature and likely precipitate.
• C)Proteins with exposed hydrophobic groups would maintain their structure and remain in solution.
• D)Both A and B would occur.
• E)Both B and C would occur.

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