Enzyme E Is Responsible for Conversion of Substrate X to Product

question 25

Multiple Choice

Enzyme E is responsible for conversion of substrate X to product U.As a result of this conversion electrons are transported to a coenzyme (FAD) within Enzyme E.In order for the reaction to be completed,a second substrate NAD⁺ must also bind Enzyme E and collect stored electrons (which converts it to product,NADH) .The graph below shows the data while varying X,with fixed concentrations of NAD⁺.What type of multi-substrate mechanism does enzyme E utilize?
Substrates: X NAD⁺
$\downarrow$$\downarrow$
Products: U NADH

• A)sequential - Ordered
• B)sequential - Random
• C)simultaneous addition
• D)Ping Pong
• E)Sequential but the data cannot differentiate between ordered and random.

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