Below Are the Steps Involved in Cloning Gene Sequences Using

question 11

Multiple Choice

Below are the steps involved in cloning gene sequences using mRNA.Arrange the steps in the appropriate order.
1) The double-stranded cDNA is treated with a restriction endonuclease to generate compatible ends for annealing and ligation.
2) mRNA is isolated from the cell and converted back into double-stranded sequences using reverse transcriptase to generate complementary DNA.
3) The RNA-DNA hybrid is treated with a nuclease to cleave the RNA strand,producing RNA fragments.
4) Reverse transcriptase completes the single-stranded cDNA when it reaches the end of the mRNA transcript.
5) RNA fragments serve as primers for DNA synthesis of the second strand of cDNA using DNA polymerase.

• A)4,2,1,5,3
• B)2,4,3,5,1
• C)2,3,5,4,1
• D)3,2,5,4,1

---

Definitions:

Payable to Cash

A term indicating that a financial instrument, like a check, is written in such a manner that it can be cashed or negotiated by any holder, not just a specific payee.

"Pay to the Order of Bearer"

A phrase used on checks or other negotiable instruments that indicates the person possessing the document has the right to cash or negotiate it.

Drawee

The party, typically a bank, required to pay the specified amount on a check or draft when it is presented for payment.

Negotiable Instrument

A written document guaranteeing the payment of a specific amount of money, either on demand or at a set time, with the payer named on the document.