Outline the steps used in the chain-termination method to determine the sequence of a region of DNA.Using the figure below,explain what occurs at steps A,B,C,D,and E. 
Definitions:
Machine Hours
A measure of the amount of time a machine is operated within a given period, used in cost accounting to allocate manufacturing overhead costs to products.
Overapplied Amount
A situation in cost accounting where the allocated factory overhead costs exceed the actual overhead costs incurred.
Factory Overhead
Indirect costs related to manufacturing, including utilities, maintenance, and factory equipment depreciation.
Machine Hours
A measure of production time, calculated as the total number of hours that machines are operated during a specific period.
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