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Below Are the Steps of a Western Blot

question 24

Multiple Choice

Below are the steps of a Western blot.What is the correct order?
1) The membrane is incubated with the secondary antibody.
2) The membrane is incubated with the primary antibody.
3) The membrane is washed with a blocking solution.
4) Proteins are transferred to a nitrocellulose membrane.
5) Protein extracts are separated by gel electrophoresis.

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Definitions:

Confidence Interval

An estimate expressed as a range that likely contains a population parameter, characterized by a lower and upper bound computed from sample data.

Two Population Means

A statistical comparison between the mean values of two distinct groups or populations.

Sample Means

The arithmetic averages derived from multiple subsets of a population, reflecting the central tendency of each subset.

Population Means

The mean values calculated from the entirety of specified populations.

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