Below Are the Steps of Solid-Phase Peptide Synthesis

question 30

Multiple Choice

Below are the steps of solid-phase peptide synthesis:
1) The protecting groups of the amino acids are removed.
2) The peptide is released from the solid support resin.
3) An incoming amino acid is activated at the carboxyl group by DCC and added to the column.
4) Fmoc is removed by treatment with a base and the C-terminal amino acid is attached to the resin molecule.
5) The resin bound C-terminal amino acid and the incoming N-terminal amino acid are coupled.What is the appropriate order of these steps?

• A)3,5,4,2,1
• B)1,4,2,5,3
• C)4,3,5,1,2
• D)2,4,5,3,1

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