You Design an Experiment in Which You Can Monitor the Step-By-Step

question 87

Essay

You design an experiment in which you can monitor the step-by-step movement of H⁺ and Ca²⁺ through the SERCA protein,starting with the protein in the E2-ATP state and moving through the transport cycle,ultimately returning to the E2-ATP state.Your control experiment includes everything required for the system to function normally,and you easily detect four distinct conformations of the protein.You then replace the ATP in the system with ATP S,a nonhydrolyzable form of ATP,and begin the experiment again with the protein in the E2-ATP state.Predict which conformations of SERCA you will detect.

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