A Mixture of Enzyme and Inhibitor Is Run Through a Size-Exclusion

question 8

Multiple Choice

A mixture of enzyme and inhibitor is run through a size-exclusion chromatography column.The activity of the enzyme is assessed before and after the chromatography.The enzyme has more activity after the chromatography step.Which of the following is true?

• A) The enzyme was not eluted fully from the column.
• B) The enzyme was denatured during chromatography.
• C) The inhibitor is a reversible inhibitor.
• D) The inhibitor is an irreversible inhibitor.

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