A Patient Is Tested for the Presence of the Factor

question 23

Multiple Choice

A patient is tested for the presence of the factor V Leiden mutation by PCR-RFLP. The mutation forms a recognition site for the MnlI restriction enzyme. Isolated DNA is amplified by PCR, and the resulting amplicons are cut with MnlI and separated by gel electrophoresis. Two bands are seen in the lane loaded with the patient's sample, one at 153 bp and the other at 67 bp. One band (220 bp) is seen in the normal control. Which of the following is the correct interpretation of these results?

• A) Normal
• B) Heterozygous mutation
• C) Homozygous mutation
• D) Wrong target and method performed for this disease

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Definitions:

Fortress Walls At Jericho

Ancient defensive structures that surrounded the city of Jericho, believed to be some of the oldest fortifications discovered to date.

BCE

stands for Before Common Era, a non-religious alternative term to B.C., denoting years before the start of the Gregorian calendar's year count.

Labor-Force Participation Rate

The ratio of working-age individuals who are either employed or on the hunt for a job.

Unemployment Rate

The fraction of the labor force that lacks employment but is keenly searching for a position.