A Patient Is Tested for the Presence of the Factor

question 23

Multiple Choice

A patient is tested for the presence of the factor V Leiden mutation by PCR-RFLP. The mutation forms a recognition site for the MnlI restriction enzyme. Isolated DNA is amplified by PCR, and the resulting amplicons are cut with MnlI and separated by gel electrophoresis. Two bands are seen in the lane loaded with the patient's sample, one at 153 bp and the other at 67 bp. One band (220 bp) is seen in the normal control. Which of the following is the correct interpretation of these results?

• A) Normal
• B) Heterozygous mutation
• C) Homozygous mutation
• D) Wrong target and method performed for this disease

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Definitions:

Specialized Niche

A specific or focused role played by an organism or entity within its environment, often referring to a narrow adaptation to a particular set of conditions.

Low Population Density

Refers to areas with few people living in them relative to their size, often measured as the number of individuals per unit area.

Reproductive Rate

The average number of offspring produced by an organism or population, influencing its ability to sustain or grow its numbers over time.

Extinct Species

Extinct species are those species of organisms that no longer exist on Earth, having completely disappeared from the biological diversity.