A PCR Assay Using Primers Specific for the Autolysin Gene

question 19

Multiple Choice

A PCR assay using primers specific for the autolysin gene (lytA) was performed on a sputum specimen from a 10-year-old girl. The specimen was positive, and all controls were acceptable. Which of the following is the best interpretation of these results?

• A) Definitive diagnosis of clinically significant Streptococcus pneumoniae pneumonia is made.
• B) Streptococcus pneumoniae is present, but clinical significance is questionable.
• C) Mycobacterium tuberculosis is detected, and the girl should be isolated.
• D) The patient has Chlamydophila pneumoniae pneumonia.

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Definitions:

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