Which Is Critical to the Success of Sequence-Specific PCR

question 22

Multiple Choice

Which is critical to the success of sequence-specific PCR?

• A) The 5  end of the primer must match the template.
• B) The forward primer must have a higher Tm than the reverse primer.
• C) The 3  base of the primer must be complementary to the template.
• D) The primers must be less than 20 bases long.

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