Which of the Following Steps Can Be Taken to Minimize

question 9

Multiple Choice

Which of the following steps can be taken to minimize mis-priming in a PCR procedure?

• A) Use high-quality deoxynucleotides, buffers, and enzymes.
• B) Seclude the polymerase from the template and primers before amplification.
• C) Load the samples carefully for gel electrophoresis of PCR products.
• D) Keep the reaction mix at room temperature prior to amplification.

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Definitions:

Outcome

The result or effect of an action, situation, or event.

Insulin

Insulin is a hormone produced by the pancreas that regulates blood glucose levels by promoting the absorption of glucose into liver, fat, and skeletal muscle cells.

Glucose

A simple sugar that is an important energy source in living organisms and is a component of many carbohydrates.

Nutrients

Substances obtained from food that are essential for maintaining bodily functions and growth, including vitamins, minerals, carbohydrates, proteins, and fats.