A PCR Assay Is Performed, and the Results Are Being

question 20

Multiple Choice

A PCR assay is performed, and the results are being analyzed. The positive control has a band at the expected size; the negative template control has a band at the same size as the positive control; the contamination control has no band; and the amplification control has a band of expected size. All of the patient samples that were run had a band the same size as the band seen in the positive and negative template control wells. How are these results interpreted?

• A) This is an acceptable run, and the patients should all be reported as positive.
• B) This run is not acceptable because the amplification control should not have a band.
• C) This run is not acceptable because the contamination control should have a band.
• D) This run is not acceptable because the negative template control should not have a band.

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Definitions:

Expected Returns

The anticipated average return of an investment over a certain period, taking into account both the probability and the impact of all possible outcomes.

Corresponding Weights

The respective importance or influence assigned to components within a mathematical formula or financial model.

Probability Distribution

A mathematical representation that enumerates all possible values and the likelihood of each for a random variable within a determined boundary.

Coefficient Of Correlation

A statistical measure that calculates the strength and direction of the relationship between two variables.