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A Hybridization Assay Is Run Under the Following Conditions: 40

question 29

Multiple Choice

A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?

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Definitions:

Marketable Securities

Financial instruments that can be easily converted into cash, often including stocks, bonds, and other securities traded on public exchanges.

Fair Value

The price that would be received for selling an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date.

Bonds Payable

Long-term liabilities representing amounts owed by an entity to bondholders, to be repaid at specified maturity dates with interest.

Net Book Value

The net book value is the value at which an asset is carried on a balance sheet, calculated as the original cost minus accumulated depreciation and impairment losses.

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